Welcome!
Chronobiosis and Energetics/Metabolism of Aging Core (CEAC)
The CEAC offers intellectual expertise specific to the design, implementation and interpretation of studies targeting the understanding of energetics and metabolic consequences and mechanisms specific to aging. The CEAC offers intellectual expertise specific to the design, implementation and interpretation of studies targeting the understanding of energetics and metabolic consequences and mechanisms that are specific to aging. Using cutting-edge technologies, the CEAC provides leadership and personnel to advise or assist investigators in performing in vivo energetics/metabolism and chronobiosis assessments as detailed below. CEAC services, which enrich and extend the studies of these researchers, are made available to E-NSC, the mini-NSC, and other NSC members at a discounted fee, and to outside investigators at a standard rate. Finally, through this Core, we will continue to develop normative physiological/functional values for healthy lifespan, and establish a plasma and tissue chronobiosis resource, which will include a discovery panel database consisting of proteomic, transcriptomic and metabolomic data from isochronic and heterochronic pairs.
Proteostasis of Aging Core
The Proteostasis of Aging Core provides state-of-the-art methodology for the study of changes in cellular protein homeostasis in aging and age-related disorders. The core performs highly specialized assays for the analysis of autophagic pathways and the ubiquitin proteasome system and provides advice on experimental design, techniques, and data interpretation in that area. For information regarding consultation fees, experimental design and methods please contact ana-maria.cuervo@einsteinmed.org.
Chronobiosis and Energetics/Metabolism of Aging Core (CEAC)
Director: Nir Barzilai, MD
Director: Derek M. Huffman, PhD
Proteostasis of Aging Core
Scientific Director: Ana Maria Cuervo, MD, PhD
Operations Manager: Antonio Diaz
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Antonio Diaz |
Operations Manager, Proteostasis of Aging Core
|
amdiazc@hotmail.com
|
||
| Ana Maria Cuervo, MD, PhD |
Scientific Director, Proteostasis of Aging Core
|
|||
| Nir Barzilai, MD |
Director, Chronobiosis and Energetics/Metabolism of Aging Core (CEAC)
|
|||
| Derek M. Huffman, PhD |
Director, Chronobiosis and Energetics/Metabolism of Aging Core (CEAC)
|
| Service list |
| ► Chronobiosis and Energetics/Metabolism of Aging (30) | |||
| Name | Description | Price | |
|---|---|---|---|
| Balance Beam | Inquire | ||
| Chronobiosis complete tissue and plasma (one set, i.e., Y-Y, Y-O, O-Y, O-O) | Inquire | ||
| Chronobiosis limited tissue request (one set, i.e., Y-Y, Y-O, O-Y, O-O) | Inquire | ||
| Chronobiosis plasma (one set, i.e., Y, Y-O, O-Y, O) ~50uL | Inquire | ||
| Chronobiosis Surgery+Housing (one set, i.e. Y-Y, Y-O, O-O) | Inquire | ||
| Chronobiosis Surgery+Housing Y-Y, Y-O, or O-O each | Inquire | ||
| Clamp assay: with radioisotope (mice) | Inquire | ||
| Clamp assay: with radioisotope (rats) | Inquire | ||
| Clamp assay: with radioisotope/C14 (mice) | Inquire | ||
| Clamp assay: with radioisotope/C14 (rats) | Inquire | ||
| Clamp assay: with radioisotope/C14 and ICV (mice) | Inquire | ||
| Clamp assay: with radioisotope/C14 and ICV (rats) | Inquire | ||
| Clamp data analysis | Inquire | ||
| Clamp surgery | Inquire | ||
| Complete dissection (weighing, fixation, freezing) | Inquire | ||
| Forced Exercise Test | Inquire | ||
| Forced Treadmill test | Inquire | ||
| Gait Analysis | Inquire | ||
| Grip Strength | Inquire | ||
| Inserting ICV or intra hypothalamic catheter | Inquire | ||
| Lipectomy | Inquire | ||
| Micro CT scan | Inquire | ||
| Mouse Metabolic Cages | Inquire | ||
| Object Placement | Inquire | ||
| Object Recognition | Inquire | ||
| Open Field | Inquire | ||
| qMR (rats or mice) | Inquire | ||
| Rat Metabolic Cages | Inquire | ||
| Rotarod | Inquire | ||
| Y Maze | Inquire | ||
| ► Proteostasis of Aging (14) | |||
| Name | Description | Price | |
| Complete Protein Turnover Analysis | Inquire | ||
| Consultation on study design, methods, interpretation | Inquire | ||
| Drug design consulting |
Computational docking setup and screening: |
Inquire | |
| Drug design consulting |
Docking analysis and data plotting: |
Inquire | |
| Em morphometric analysis of autophagic compartments*** | Inquire | ||
| KFERQ-Dendra - High content microscopy/96 well plate | Inquire | ||
| LC3 flux - immunoblot (8 conditions) | Inquire | ||
| Lysosomal compartment – Lysotracker/ 8 well | Inquire | ||
| Lysosomal markers (panel) - Immunoblot | Inquire | ||
| mCherry-GFP-LC3 High content microscopy/96 well plate | Inquire | ||
| Mitophagy - immunofluorescence colocalization/8 well | Inquire | ||
| Protein degradation (1 type sample 6 conditions with triplicates) | Inquire | ||
| Protein synthesis (4 samples with triplicates) | Inquire | ||
| Training in proteostasis analysis procedures | Inquire | ||
| Name | Price | |
|---|---|---|
