Molecular Biology and Next Generation Technology / Hepatic Animal Models, Stem Cells, and Cell Therapy Core

Molecular Biology and Next Generation Technology Core Overview and Services

The Molecular Biology and Next Generation Technology Core provides a wide range of unique services to the Einstein community. In a new emphasis, the core is dedicated to bringing state of the art methods in microRNA (miRNA) research to biomedical scientists with all levels of expertise. The core provides services ranging from classical qPCR and RT-qPCR plus specialized preparation of miRNA and development of targeted genotyping assays.

• qPCR and RT-qPCR services; training and use of new ABI 7500 Fast Real-Time PCR System; help with assay development
• Fujifilm FLA 5100 scanner for radioautography, fluorescence or chemifluorescence scan of Southern, Northern and Western Blots  
• FluorChemQ imaging of agarose or polyacrylamide gels, protein, and nucleic acids blots probed with fluorescence or chemiluminescence
• MicroRNA profiling using custom made miRNA microarrays plus help with data analysis
• NanoDrop2000 spectrophotometer, Gene Gnome Syngene Bio imager and BMG Fluoristar Optima  instruments
• Help with classic DNA, cDNA and miRNA cloning
• Library preparation for next generation sequencing for both Illumina and Ion Torrent instruments
• Help with exosome studies and with characterization of microbiome
• Core interactions with Next Generation Sequencing Facilities

Hepatic Animal Models, Stem Cells, and Cell Therapy Core Overview and Services

This Core breeds unique animals of interest, reproduces animal models, provides training in procedures and consultation in experimental design.  New technologies are developed for animal and human stem cell and cell therapy studies. Primary animal or human liver cells of consistently high quality are isolated and distributed. Liver cells generated from pluripotent stem cells with highly efficient differentiation protocols can also be provided.  Animal use requires ACUC approval for the investigator’s project. Disease models include analbuminemic rat, Gunn rat, LEC rat and Atp7b-/- mice (Copper toxicosis),  and hemophilia A mice, along with additional rat and mouse models for cell transplantation research.  The Core offers training in specialized liver procedures, e.g., partial hepatectomy, bile duct ligation or cannulation, portal vein cannulation, liver perfusion, induction of liver injury or fibrosis, etc. The Core can provide specialized cell culture media and additives for hepatocytes.
Services

• Bred animals
• Isolation and culture of animal and human liver cells
• Distribution of liver cells generated from pluripotent stem cells
• Provision of cell culture additives and materials
• Reproduction of established animal models
• Training in animal procedures

Leadership

Animal Models, Stem Cells and Cell Therapy

Scientific Director
Sanjeev Gupta, M.D.

Operations Manager
David Neufeld, Ph.D.

Molecular Biology and Next Generation Technology

Scientific Director
David Q.H. Wang, M.D., Ph.D.

Scientific Co-Director
Robert Burk, M.D.

Operations Director
Tatyana Tchaikovskaya, Ph.D.

Links and Resources

1. Molecular Biology and Next Generation Technology Core Website
2. Hepatic Animal Models, Stem Cells, and Cell Therapy Core Website

Contacts