Stable Isotope and Metabolomics Core

Please see our website for more information about the pertinent metabolic pathways for this module.

Please see our website for more information about the pertinent metabolic pathways for this module.

The pump contains 50 mg of [U-13C6] glucose, each dissolved in 200 uL of water. The mini-pump is inserted in the subcutaneous space, and therefore infusion conditions approximate those of the venous-arterial (V-A) mode of infusion and sampling. Mass isotopomer distribution analysis is used for calculation of glucose production and recycling.

Reference:

Xu, J., Xiao, G., Trujillo, C., Chang, V., Blanco, L., Chung, B. Bassilian, S., Saad, M., Tontonoz, P., Lee, W-N. P. and Kurland, I.J. (2002) PPAR alpha Influences Gluconeogenic Substrate Utilization for Hepatic Glucose Production: The Glucose Metabolome of the PPAR alpha Null Mouse. J Biol Chem. 277(52):50237-50244, PMID: 12176975

Consultation for use and interpretation of multivariate analysis on metabolomic and lipidomic datasets.

Please see our website for more information about the pertinent metabolic pathways for this module.

Please see our website for more information about the pertinent metabolic pathways for this module.

Bile acids are synthesized from cholesterol through both classical and alternative pathways. In the alternative pathway, the side chain oxidation of cholesterol precedes the steroid ring modifications, first yielding 24-, 25-, and 27-hydroxycholesterol metabolites, opposite to the process in the classical pathway. The alternative and classical pathway bile acids share the primary bile acid chenodeoxycholic acid, with 12-a-hydroxylation of chenodeoxycholic acid via CYP8B1to cholic acid. Modifications of bile acids can affect their properties and their ability to activate bile acid receptors. Dysregulation of bile acid synthesis can be seen in inborn errors of metabolism, insulin resistance, hepatocellular Ca and chronic ethanol consumption. Perturbations in the microbiome also affect bile acid pool size and composition (see references below). This panel surveys conditions of bile acid dysregulation. Please see our website for more information about the pertinent metabolic pathways for this module.

This module includes acetyl CoA, malonyl CoA, and succinyl CoA, in order to interface with acyl carnitine analysis in Module #1, and TCA cycle measurements in Modules # 3 and 7.

GC/MS will identify about 80 to 120 small metabolites based on the difference of samples, in the combined glycolytic/gluconeogenic, pentose and TCA cycle pathways. For an example by the SIMC facility, see:

Vaitheesvaran B, Yang L, Hartil K, Glaser S, Yazulla S, et al. (2012) Peripheral Effects of FAAH Deficiency on Fuel and Energy Homeostasis: Role of Dysregulated Lysine Acetylation. PLoS ONE 7(3): e33717. doi:10.1371/journal.pone.0033717

Please see our website for more information about the pertinent metabolic pathways for this module.

Both fatty acid and cholesterol synthesis can be assessed in tissues, depending on the time frame for the study. For examples by the SIMC Facility see:

1) Haas et al, Hepatic Insulin Signaling Is Required for Obesity-Dependent Expression of SREBP-1c mRNA but Not for Feeding-Dependent Expression, Cell Metabolism 15, 873–884, 2012
2) Zhao et al Regulation of lipogenesis by cyclin-dependent kinase 8–mediated control of SREBP-1, J. Clin. Invest. 122:2417-27, 2012
3) Vaitheesvaran B, Yang L, Hartil K, Glaser S, Yazulla S, et al. (2012) Peripheral Effects of FAAH Deficiency on Fuel and Energy Homeostasis: Role of Dysregulated Lysine Acetylation. PLoS ONE 7(3): e33717

The targeted metabolomics approach in this assay is based on measurements with the AbsoluteIDQ p180 kit (BIOCRATES Life Sciences AG, Innsbruck, Austria). This method allows simultaneous quantification of 188 metabolites in plasma, tissues or cell pellets using liquid chromatography and flow injection analysis–mass spectrometry. For an example of this module’s utility, see: Wang-Sattler et al Novel biomarkers for pre-diabetes identified by metabolomics, Molecular Systems Biology 8; 615; doi:10.1038/msb.2012.43. Please see our website for more information about the pertinent metabolic pathways for this module.

(Yeast & Bacteria)

Choline and betaine are essential nutrients. Choline is important for brain function, liver health, reproduction, and fetal and infant development. Betaine acts as a methyl donor in the liver and as an important osmolyte to protect the cells of medulla in the kidney. Their gut microbial metabolite trimethylamine N-oxide (TMAO) was considered non-toxic, but it recently has been associated with increased risk for cardiovascular disease. This finding has stimulated a growing interest in analyzing these compounds in bio fluids and studying their associations with human health and diseases. This assay allows simultaneous quantification of free choline, betaine, TMAO and creatinine in plasma, urine or tissue samples. The analysis is performed using liquid chromatography-stable isotope dilution-multiple reaction monitoring mass spectrometry (LC-SID-MRM/MS). Please see our website for more information about the pertinent metabolic pathways for this module.

Covers staff overtime for cleaning and setup after business hours.

Covers staff overtime for cleaning and setup for weekend use.

Note: This is only the price for the plate and reagents. Seahorse services are priced at an hourly rate plus the cost of the plate and reagents.

Note: This is only the price for the plate and reagents. Seahorse services are priced at an hourly rate plus the cost of the plate and reagents.

The targeted metabolomics approach in this assay is based on measurements with the AbsoluteIDQ p180 kit (BIOCRATES Life Sciences AG, Innsbruck, Austria). This method allows simultaneous quantification of 188 metabolites in plasma, tissues or cell pellets using liquid chromatography and flow injection analysis–mass spectrometry. For an example of this module’s utility, see: Wang-Sattler et al Novel biomarkers for pre-diabetes identified by metabolomics, Molecular Systems Biology 8; 615; doi:10.1038/msb.2012.43. Please see our website for more information about the pertinent metabolic pathways for this module.

Choline and betaine are essential nutrients. Choline is important for brain function, liver health, reproduction, and fetal and infant development. Betaine acts as a methyl donor in the liver and as an important osmolyte to protect the cells of medulla in the kidney. Their gut microbial metabolite trimethylamine N-oxide (TMAO) was considered non-toxic, but it recently has been associated with increased risk for cardiovascular disease. This finding has stimulated a growing interest in analyzing these compounds in bio fluids and studying their associations with human health and diseases. This assay allows simultaneous quantification of free choline, betaine, TMAO and creatinine in plasma, urine or tissue samples. The analysis is performed using liquid chromatography-stable isotope dilution-multiple reaction monitoring mass spectrometry (LC-SID-MRM/MS). Please see our website for more information about the pertinent metabolic pathways for this module.

Please see our website for more information about the pertinent metabolic pathways for this module.

Please see our website for more information about the pertinent metabolic pathways for this module.

Bile acids are synthesized from cholesterol through both classical and alternative pathways. In the alternative pathway, the side chain oxidation of cholesterol precedes the steroid ring modifications, first yielding 24-, 25-, and 27-hydroxycholesterol metabolites, opposite to the process in the classical pathway. The alternative and classical pathway bile acids share the primary bile acid chenodeoxycholic acid, with 12-a-hydroxylation of chenodeoxycholic acid via CYP8B1to cholic acid. Modifications of bile acids can affect their properties and their ability to activate bile acid receptors. Dysregulation of bile acid synthesis can be seen in inborn errors of metabolism, insulin resistance, hepatocellular Ca and chronic ethanol consumption. Perturbations in the microbiome also affect bile acid pool size and composition (see references below). This panel surveys conditions of bile acid dysregulation. Please see our website for more information about the pertinent metabolic pathways for this module.

This module includes acetyl CoA, malonyl CoA, and succinyl CoA, in order to interface with acyl carnitine analysis in Module #1, and TCA cycle measurements in Modules # 3 and 7.

GC/MS will identify about 80 to 120 small metabolites based on the difference of samples, in the combined glycolytic/gluconeogenic, pentose and TCA cycle pathways. For an example by the SIMC facility, see:

Vaitheesvaran B, Yang L, Hartil K, Glaser S, Yazulla S, et al. (2012) Peripheral Effects of FAAH Deficiency on Fuel and Energy Homeostasis: Role of Dysregulated Lysine Acetylation. PLoS ONE 7(3): e33717. doi:10.1371/journal.pone.0033717

Please see our website for more information about the pertinent metabolic pathways for this module.

Both fatty acid and cholesterol synthesis can be assessed in tissues, depending on the time frame for the study. For examples by the SIMC Facility see:

1) Haas et al, Hepatic Insulin Signaling Is Required for Obesity-Dependent Expression of SREBP-1c mRNA but Not for Feeding-Dependent Expression, Cell Metabolism 15, 873–884, 2012
2) Zhao et al Regulation of lipogenesis by cyclin-dependent kinase 8–mediated control of SREBP-1, J. Clin. Invest. 122:2417-27, 2012
3) Vaitheesvaran B, Yang L, Hartil K, Glaser S, Yazulla S, et al. (2012) Peripheral Effects of FAAH Deficiency on Fuel and Energy Homeostasis: Role of Dysregulated Lysine Acetylation. PLoS ONE 7(3): e33717

Please see our website for more information about the pertinent metabolic pathways for this module.

Please see our website for more information about the pertinent metabolic pathways for this module.

The pump contains 50 mg of [U-13C6] glucose, each dissolved in 200 uL of water. The mini-pump is inserted in the subcutaneous space, and therefore infusion conditions approximate those of the venous-arterial (V-A) mode of infusion and sampling. Mass isotopomer distribution analysis is used for calculation of glucose production and recycling.

Reference:

Xu, J., Xiao, G., Trujillo, C., Chang, V., Blanco, L., Chung, B. Bassilian, S., Saad, M., Tontonoz, P., Lee, W-N. P. and Kurland, I.J. (2002) PPAR alpha Influences Gluconeogenic Substrate Utilization for Hepatic Glucose Production: The Glucose Metabolome of the PPAR alpha Null Mouse. J Biol Chem. 277(52):50237-50244, PMID: 12176975

Consultation for use and interpretation of multivariate analysis on metabolomic and lipidomic datasets.

(Yeast & Bacteria)

Covers staff overtime for cleaning and setup after business hours.

Covers staff overtime for cleaning and setup for weekend use.

Note: This is only the price for the plate and reagents. Seahorse services are priced at an hourly rate plus the cost of the plate and reagents.

Note: This is only the price for the plate and reagents. Seahorse services are priced at an hourly rate plus the cost of the plate and reagents.