Human Preclinical Modeling and Surveillance Core

Overview

The Human Preclinical Modeling and Surveillance Core Facility is a unified, state-of-the-art platform that brings together specialized units in Stem Cell Isolation, Xenotransplantation, Patient Derived Xenograft (PDX) Models, Organoids, Induced Pluripotent Stem Cells (iPSC), and Gene Editing under one integrated research ecosystem. The institute provides advanced infrastructure and technical expertise and consultation services to support high impact studies in regenerative medicine, cancer biology, developmental science, and precision therapeutics. By seamlessly connecting complementary model systems from cellular engineering and organoid platforms with in vivo xenograft and PDX models it enables rigorous, physiologically relevant investigation of human disease and therapeutic response.

Units

• Stem Cell Isolation, Xenotransplantation and PDX Unit: Mrs. Swath-Rao Narayanagari, Technical Director
• Organoid Innovation and Disease Modeling Unit: Dr. Nityanand Srivastava, Technical Director
• iPSC Unit: Dr. Hager Mansour, Technical Director
• Gene Editing Unit: Dr. Yongwei Zhang, Technical Director
• Data Mining and Diagnostics Unit: Ms. Colette Prophete, Co-Technical Director and Mr. Varun Gupta, Co-Technical Director

Services

• Stem Cell Isolation, Xenotransplantation and PDX Unit
  • Multiparameter flow cytometry and cell sorting
  • Isolation and characterization of stem, progenitor, and tumor-initiating cells
  • Development and propagation of cell line– and patient-derived xenograft models
  • In vivo efficacy studies and compound administration
  • Rodent toxicology and maximum tolerated dose studies
  • Blood, bone marrow, and tumor sample collection
  • PDX model generation, expansion, cryopreservation, and distribution
  • Unit website
• Organoid Innovation and Disease Modeling Unit
  • Generation, expansion, characterization, and biobanking of organoid models
  • Development of patient-derived, engineered, and humanized 3D culture systems
  • Organoid-based disease modeling and therapeutic testing
  • Drug-response, toxicity, and resistance assays
  • Live-cell, confocal, and high-content imaging of cells, spheroids, and organoids
  • Experimental design, protocol development, and assay optimization
  • Unit website
• iPSC Unit
  • Generation, expansion, validation, and banking of human iPSC lines
  • Pluripotency, genomic-stability, and differentiation-potential testing
  • Differentiation into hematopoietic, neuronal, cardiac, hepatic, and immune cell types
  • Development of patient-derived and genome-edited disease models
  • Training and consultation in iPSC culture, characterization, and differentiation
  • Unit website
• Gene Editing Unit
  • Constitutive and conditional gene knockout
  • Precision gene knock-in
  • CRISPR interference and CRISPR activation
  • Base editing and prime editing
  • Genetic modification of iPSC and organoid models
  • Experimental design and gene-editing consultation
  • Unit website
• Data Mining and Diagnostics Unit
  • Analysis and integration of research and clinical datasets
  • Confocal microscopy and training for bone and bone marrow imaging
  • In vivo bioluminescence and fluorescence imaging
  • Preclinical ultrasound imaging
  • Complete hematology analysis of peripheral blood and bone marrow
  • Multispecies blood-cell counting and characterization
  • Unit website

Leadership

Scientific Director
Winfried Edelmann, M.D., Ph.D.
718-678-1086
winfried.edelmann@einsteinmed.edu

Location

Links and Resources

1. Core Website

Contacts

Available Equipment and Resources