Human Preclinical Modeling and Surveillance Core

Overview

The Human Preclinical Modeling and Surveillance Core (formerly the Stem Cell Isolation, Xenotransplantation, and Diagnostics Core) advances stem cell and cell therapeutic research by providing key technological support for human stem cells FACS sorting and transplantation services to test stem cell differentiation potential in vivo. The core is comprised of five units that work together to provide investigators with comprehensive research support.

Units

• Human (Stem) Cell Isolation and Xenotransplantation Unit: Mrs. Swath-Rao Narayanagari, Technical Director
• Organoid Models Unit: Dr. Nityanand Srivastava, Technical Director
• iPSC Unit: Dr. Hager Mansour, Technical Director
• Gene Targeting Unit: Dr. Yongwei Zhang, Technical Director
• Diagnositcs Unit: Colette Prophete, Technical Director

Services

Flow cytometry core facility:

• Multiparameter stem cell isolation
• Assays to test self-renewal capacity and pluripotency of stem cells
• Assays to test cancer-initiating potential of putative cancer stem cells

Xeno core transplantation facility:

• Xenograft studies:

          Establish Xenograft models from cell lines and patient samples provided by investigators. Methodologies for cell implantation include subcutaneous, intraperitoneal, intravenous (retro orbital, tail vein 
          routes), intra femoral, neonatal intracardiac and neonatal intra hepatic. Successfully engrafted tumors are propagated by serial transplantations or and used for further drug studies.

• Efficacy studies:

          Evaluate in vivo efficacy of agents either alone or in combination with standard chemotherapy regimens against murine tumors, human tumor xenografts and transgenic mouse models of human cancer.

• Compound delivery:

          Formulated compounds for in vivo delivery using variety of commonly used vehicles and are administered by number of routes, including oral gavage, intraperitoneal, intravenous and by food and/or drinking water.

• Rodent toxicology:

          Closely work with principal investigators to determine max-tolerated dose after single dose, or repeat-dose toxicity studies in novel drug applications for cancer treatments on rodents.

• Sample collection:

           Live bone marrow sampling, blood and tumor samples at multiple time points to assess engraftment ability, drug treatment efficiency.

Leadership

Scientific Director
Winfried Edelmann, M.D., Ph.D.
718-678-1086
winfried.edelmann@einsteinmed.edu

Location

Links and Resources

1. Core Website

Contacts

Available Equipment and Resources