Welcome!
Animal Behavioral Core
The Animal Behavioral Evaluation Core, supported by the Dominick P. Purpura Department of Neuroscience and the Rose F. Kennedy Intellectual and Developmental Disabilities Research Center, provides expert staff and equipment for testing the behavior of mice and rats. These tests are broadly applicable to the study of heritable and developmental disorders of cognitive function, sensorimotor disorders, psychiatric and affective disorders, metabolic disorders, cancer, neuropathological and infectious diseases, aging and reproductive function. Services include:
Cellular and Molecular Shared Equipment Facility
The Kennedy Center Cellular and Molecular Shared Equipment Facility on the third floor, provides access to common experiment equipment (including western blot and gel scanners, scintillation counter, film developer and various shakers and centrifuges).
Tissue Engineering and Cellular Reprogramming Core
The Tissue Engineering and Cellular Reprogramming Core provides consultation, equipment, facilities and technical support for the generation and reprogramming of a wide range of viable embryonic and neural cell types in culture and in vivo and the analysis of integrative cellular, molecular and functional properties generated from these experimental paradigms. The core houses 2 six-foot and 1 four-foot Edgegard TC Laminar Flow Hoods, 2 Steril Gard III Biosafety Hoods, P2 facility, an EMS OTS-4000 Vibratome and 12 Forma Scientific Water Jacketed Tissue Culture Incubators equipped with separate O2, CO2, N2 and additional gaseous mixture and temperature controls to support these services.
| Cellular and Molecular Shared Equipment Facility | Animal Behavioral Core |
Tissue Engineering and Cellular Reprogramming Core |
| Director: Kevin Fisher | Director: Mikhail Kislin, Ph.D. | Director: Solen Gokhan, M.D. |
| Hours | Locations |
|
Varies, contact core director |
Rose F. Kennedy Center |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Kevin Fisher |
Director, Cellular and Molecular Shared Equipment Facility
|
718-430-8945
|
kevin.fisher@einsteinmed.edu
|
|
| Mikhail Kislin, Ph.D. |
Director, Animal Behavioral Core
|
718-430-8522
|
mikhail.kislin@einsteinmed.edu
|
|
| Sheela Devakanmalai, Ph.D. |
Cell and Tissue Culture
|
718-430-4229
|
g.devakan@einsteinmed.edu
|
|
| Solen Gokhan, M.D. |
Director, Tissue Engineering and Cellular Reprogramming Core
|
718-430-3542
|
solen.gokhan@einsteinmed.edu
|
| Service list |
| ► Gene Gun (2) | |||
| Name | Description | Price | |
|---|---|---|---|
| Gene Bullets (~30 per set) - Assisted | Inquire | ||
| Gene Bullets (~30 per set) - Unassisted | Inquire | ||
| ► Graphic Design & Printing (2) | |||
| Name | Description | Price | |
| Graphic design | Inquire | ||
| Poster printing | Inquire | ||
| ► Cell and Tissue Culture (18) | |||
| Name | Description | Price | |
| C02 Tissue Culture Incubator Room Fee (TECR) | Inquire | ||
| Consultation - First Hour (TECR) | Inquire | ||
| Consultation (TECR) |
Over one hour |
Inquire | |
| Embryonic Rodent Ultrasound Guided Implantation Assisted Session (TECR) | Inquire | ||
| Embryonic Rodent Ultrasound Guided Implantation Hourly Rate (TECR) |
This rate is applied if a session goes beyond three hours. |
Inquire | |
| Embryonic Rodent Ultrasound Guided Implantation Training Session (TECR) | Inquire | ||
| Embryonic Rodent Ultrasound Guided Implantation Unassisted Session (TECR) | Inquire | ||
| Liquid Nitrogen Repository - Per Box/Month (TECR) | Inquire | ||
| Murine ES Cell Line Procurement Service (TECR) | Inquire | ||
| PCR Plate (TECR) | Inquire | ||
| PCR Plate - Bulk Rate (TECR) |
This rate is available to PI labs after paying for 25 plates at the regular rate in a calendar year. |
Inquire | |
| Preparation and maintenance of basic in vitro formats (TECR) | Inquire | ||
| Preparation and maintenance of specific developmental cell types (TECR) | Inquire | ||
| Preparation and manipulation of pluripotent and related cellular paradigms: iPS, direct somatic lines; and organoids (TECR) | Inquire | ||
| Stereotactic Rodent Brain Surgery Assisted Session (TECR) | Inquire | ||
| Stereotactic Rodent Brain Surgery Hourly Rate (TECR) |
This rate is applied if a session goes beyond three hours. |
Inquire | |
| Stereotactic Rodent Brain Surgery Training Session (TECR) | Inquire | ||
| Stereotactic Rodent Brain Surgery Unassisted Session (TECR) | Inquire | ||
| ► Analysis and Computation (2) | |||
| Name | Description | Price | |
| Analysis and Computation | Inquire | ||
| Destriping and Stitching | Inquire | ||
| ► Assistance Surcharges (19) | |||
| Name | Description | Price | |
| Assistance Surcharge - Axioscan | Inquire | ||
| Assistance Surcharge - Brucker Upright Multiphoton | Inquire | ||
| Assistance Surcharge - BSL2 Stereotactic Injection Facility | Inquire | ||
| Assistance Surcharge - Cryostar NX70 Cryostat | Inquire | ||
| Assistance Surcharge - Flexstation Flourescence Plate Reader | Inquire | ||
| Assistance Surcharge - Helios Gene Gun Shot Station | Inquire | ||
| Assistance Surcharge - Image Analysis and Graphics Mac Workstation | Inquire | ||
| Assistance Surcharge - Image Analysis and Graphics PC Workstation | Inquire | ||
| Assistance Surcharge - Leica CM3050S Cryostat | Inquire | ||
| Assistance Surcharge - Leica VT1000S Vibratome | Inquire | ||
| Assistance Surcharge - Olympus Fluoview 500 Laser Scanning Confocal Microscope | Inquire | ||
| Assistance Surcharge - Olympus Inverted Multi-Time, Multi-Sample Automated Widefield Epiflourescence Microscope | Inquire | ||
| Assistance Surcharge - Olympus Upright Widefield Epiflourescence Microscope | Inquire | ||
| Assistance Surcharge - Olympus Upright Widefield Neurolucida/Stereoinvestigator Microscope | Inquire | ||
| Assistance Surcharge - SmartSpim Light Sheet Microscope Image Acquisition | Inquire | ||
| Assistance Surcharge - SmartSpim Light Sheet Microscope Processing | Inquire | ||
| Assistance Surcharge - Veritas Laser Microdissection and Laser Capture System | Inquire | ||
| Assistance Surcharge - Zeiss Duo V2 Inverted Laser Scanning Confocal Microscope | Inquire | ||
| Assistance Surcharge - Zeiss LSM880 Airyscan Confocal Microscope with Super-Resolution | Inquire | ||
| ► Animal Behavioral Services (3) | |||
| Name | Description | Price | |
| Assisted Hourly Rate (Animal Behavioral Core Services) | Inquire | ||
| Training Hourly Rate (Animal Behavioral Core Services) | Inquire | ||
| Unassisted Hourly Rate (Animal Behavioral Core Services) | Inquire | ||
| ► Macro/Script (1) | |||
| Name | Description | Price | |
| Macro/Script Writing | Inquire | ||
| ► Common Equipment (3) | |||
| Name | Description | Price | |
| Common Equipment Usage Fee | Inquire | ||
| Viewer Software Dongle Rental - Weekdays | Inquire | ||
| Viewer Software Dongle Rental - Weekend |
Rate for Saturday + Sunday. |
Inquire | |
| ► Equipment Surcharge (1) | |||
| Name | Description | Price | |
| Equipment surcharge - Airyscan surcharge | Inquire | ||
| ► Light Sheet Microscopy Clearing Service (3) | |||
| Name | Description | Price | |
| Clearing Service Large Sample |
Specimen examples are rat brain or skull-on mouse brain. The service includes SHIELD processing to better preserve integrity and original volume. |
Inquire | |
| Clearing Service Small Sample |
Specimen examples include mouse long bone or artery. Typically specimens with one dimension 1mm or less. The service includes SHIELD processing to better preserve integrity and original volume. |
Inquire | |
| Clearing Service Standard Sample |
Examples of sample are a mouse brain or mouse partial liver lobe. The service includes SHIELD processing to better preserve integrity and original volume. |
Inquire | |
| ► Light Sheet Microscopy Processing Add-ons (2) | |||
| Name | Description | Price | |
| Light Sheet Microscope Data Destriping Adjustment |
Adjustment in fees for non-standard de-striping run such as failed original initiation |
Inquire | |
| LSM Data Imaris Formatting |
Destriped Light Sheet Microscope data will be converted to format compatible for use in Imaris 3D visualization software |
Inquire | |
| ► Light Sheet Microscopy RI Matching and Sample Mounting (3) | |||
| Name | Description | Price | |
| RI Matching Training |
Explanation of how best to match your specimen to the desired Refractive Index solution for Imaging. Includes 10 ml of Easy Index typically used for the Core's Light Sheet Microscope. |
Inquire | |
| Sample Embedding and Mounting Assistance |
Assistance with embedding and mounting additional samples for trained individuals |
Inquire | |
| Sample Embedding and Mounting Training |
Hands-on demonstration with one user specimen on embeding and mounting sample on carrier for imaging with Core's Light Sheet Microscope. Includes staff preparation of needed heated agarose-EI solution |
Inquire | |
| ► Light Sheet Microscopy Reagents and Accessories (26) | |||
| Name | Description | Price | |
| Agarose, ultra low-melting | Inquire | ||
| Delipidation Buffer | Inquire | ||
| Easy Index per 500 ml bottle | Inquire | ||
| Easy Index per ml | Inquire | ||
| EI Embedding Medium |
This is a semi-dissolved solution of ultra-low melting agarose in Easy Index for embedding cleared samples typically for Light Sheet Imaging on the Core's microscope |
Inquire | |
| Embedded Sample Holders for LSM - Delrin Standard Large As Is |
Delrin fabricated holder designed for agarose gel embedded rat brain or similar sized specimens. |
Inquire | |
| Embedded Sample Holders for LSM - Delrin Standard Large Ready to Use |
Delrin fabricated holder designed for agarose gel embedded rat brain or similar sized specimens. Ready to use (Cleaned, velcro strip holder, and temporary thermal walls to retain liquid gel) |
Inquire | |
| Embedded Sample Holders for LSM - Delrin Standard Small As Is |
Delrin fabricated holder designed for agarose gel embedded mouse brain or similar or smaller sized specimens. |
Inquire | |
| Embedded Sample Holders for LSM - Delrin Standard Small Ready to Use |
Delrin fabricated holder designed for agarose gel embedded mouse brain or similar or smaller sized specimens. Ready to use (Cleaned, velcro strip holder, and temporary thermal walls to retain liquid gel) |
Inquire | |
| Embedded Sample Holders for LSM - Homemade Large As Is |
3D Printed in-house holder designed for agarose gel embedded rat brain or similar sized specimens. |
Inquire | |
| Embedded Sample Holders for LSM - Homemade Large Ready to Use |
3D Printed in-house holder designed for agarose gel embedded rat brain or similar sized specimens. Ready to use (Cleaned, velcro strip holder, and temporary thermal walls to retain liquid gel) |
Inquire | |
| Embedded Sample Holders for LSM - Homemade Small As Is |
3D Printed in-house holder designed for agarose gel embedded mouse brain or similar sized specimens. |
Inquire | |
| Embedded Sample Holders for LSM - Homemade Small Ready to Use |
3D Printed in-house holder designed for agarose gel embedded mouse brain or similar sized specimens. Ready to use (Cleaned, velcro strip holder, and temporary thermal walls to retain liquid gel) |
Inquire | |
| Light Sheet Microscopy Miscellaneous Items |
For purchase of light sheet microscopy related non-standard reagents and accessories |
Inquire | |
| Mesh Bag Set Large |
Mesh bags to hold specimens for active clearing and labeling. Sizes suitable e.g. for whole rat brain, or for whole mouse brain with skull on |
Inquire | |
| Mesh Bag Set Small |
Mesh bags to hold specimens for active clearing and labeling. Sizes suitable for e.g. whole mouse brain |
Inquire | |
| Primary Device Buffer | Inquire | ||
| Primary Sample Buffer | Inquire | ||
| Propidium Iodide Solution | Inquire | ||
| Radiant Buffer | Inquire | ||
| Radiant Catalyst | Inquire | ||
| Sample Bag Ring Holder and Lock |
Holder needed to clear or label multiple samples in SmartBatch+ Device |
Inquire | |
| Secondary Device Buffer | Inquire | ||
| Secondary Sample Buffer | Inquire | ||
| Staining Cup, Batch | Inquire | ||
| SYTOX Deep Red Dye Solution | Inquire | ||
| ► Light Sheet Microscopy SHIELD Service (4) | |||
| Name | Description | Price | |
| SHIELD Service Large Sample |
This service performs SHIELD processing on user's paraformaldehyde-fixed specimens to better preserve integrity and volume for additional treatments such as for clearing protocols. Samples here include a rat brain, skull-on mouse brain and similar sized specimens. |
Inquire | |
| SHIELD Service Small Sample: 24-well multiwell formats (base price) |
This service performs SHIELD processing on user's paraformaldehyde-fixed specimens to better preserve integrity and volume for additional treatments such as for clearing protocols. Samples here include very small specimens such as tissue slices of mouse brain that are performed in 24-well multiwell plates. No more than 3 coronal slices per well are recommended e.g. of adult mouse brain. |
Inquire | |
| SHIELD Service Small Sample: 24-well multiwell formats (supplemental charge per well) |
This service performs SHIELD processing on user's paraformaldehyde-fixed specimens to better preserve integrity and volume for additional treatments such as for clearing protocols. Samples here include very small specimens such as tissue slices of mouse brain that are performed in 24-well multiwell plates. No more than 3 coronal slices per well are recommended e.g. of adult mouse brain. This entry based on number of wells involved covers expenses for reagents and addiitonal handling labor needed. |
Inquire | |
| SHIELD Service Standard Sample |
This service performs SHIELD processing on user's paraformaldehyde-fixed specimens to better preserve integrity and volume for additional treatments such as for clearing protocols. Samples here include a mouse brain or smaller sized specimens. |
Inquire | |
| ► unclassified (1) | |||
| Name | Description | Price | |
| Easy Index per 500 ml bottle | Inquire | ||
| ► Behavioral Core - Testing, do not use! (2) | |||||||||||||||||||||||||||||||||||||||||||||||||||
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| ► Cell and Tissue Preparation (15) | |||||||||||||||||||||||||||||||||||||||||||||||||||
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| ► Helios Gene Gun (2) | |||||||||||||||||||||||||||||||||||||||||||||||||||
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| ► In Vivo Cell and Vector Delivery/Recovery (2) | |||||||||||||||||||||||||||||||||||||||||||||||||||
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| ► Micropipettes (1) | |||||||||||||||||||||||||||||||||||||||||||||||||||
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| ► Neuroscience Stereotactic Instruments (4) | |||||||||||||||||||||||||||||||||||||||||||||||||||
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| ► Plate Reader (1) | |||||||||||||||||||||||||||||||||||||||||||||||||||
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| ► QRT PCR (2) | |||||||||||||||||||||||||||||||||||||||||||||||||||
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| ► Workstations (5) | |||||||||||||||||||||||||||||||||||||||||||||||||||
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| ► Unclassified (16) | |||||||||||||||||||||||||||||||||||||||||||||||||||
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