Welcome!
The Flow Cytometry Core Facility provides a broad range of equipment and services for the analysis and isolation of cells and other similar-sized particles based on fluorescent labeling. Four high-speed cell sorters are available to enable optimized sorting of highly purified cells, subcellular organelles or bacteria. The facility also supports a variety of analytical flow cytometers capable of performing basic to advanced multiparameter fluorescence analysis of many types of cell suspensions as well as analysis of intracellular signaling. An experienced staff of technical experts provides instruction in the design, execution and analysis of flow cytometry-based studies.
Scientific Director: Steve Porcelli, M.D.
Operations Director: Jinghang Zhang, M.D.
| Hours | Location |
|
Monday – Friday 9:00 a.m. – 5:00 p.m.
All analyzers and some cell sorters are open 24/7 for self operators |
Flow Cytometry Core Facility
Chanin Building: Room 307- 310
Price Center: Room 159
Albert Einstein College of Medicine
1300 Morris Park Ave
Bronx, NY 10461
|
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Jinghang Zhang, M.D. |
Operations Director
|
(718) 430-8787
|
jinghang.zhang@einsteinmed.edu
|
Chanin 309
|
| Aodeng |
Technician
|
(718) 430-4176
|
fnu.aodengtuya@einsteinmed.edu
|
Chanin 309
|
| Yu Zhang (Joey), Ph.D. |
Assistant Operations Director
|
(718) 430-4164 or (718)678-1141
|
yu.zhang@einsteinmed.edu
|
Chanin 309 or Price 159
|
| Ming Liu |
Technician
|
(718) 430-4163
|
ming.liu@einsteinmed.edu
|
Chanin 307B or Chanin 309
|
| Fixed Cost Services |
| ► Flow Cytometry Core Services (2) | |||
| Name | Description | Price | |
|---|---|---|---|
| FlowJo License (Yearly Fee) | Inquire | ||
| FlowJo License (Per Quarter) |
FlowJo Licenses are priced based on quarterly use of the software. |
Inquire | |
| ► Immune Monitoring Core Services (12) | |||
| Name | Description | Price | |
| Akoya 5-Plex, 6-Color MOTiF™ panel development (IMC) |
Core staff will create an assay to stain FFPE slides with 5 antibodies on separate fluorescent channels. User will provide primary antibodies and sufficient slides to develop the staining panel. |
Internal
$2,575.00
each
External $4,120.00 each External Affiliate $3,347.50 each |
|
| Akoya 6-Plex, 7-Color MOTiF™ panel development (IMC) |
Core staff will create an assay to stain FFPE slides with 6 antibodies on separate fluorescent channels. User will provide primary antibodies and sufficient slides to develop the staining panel. |
Internal
$3,090.00
each
External $4,944.00 each External Affiliate $4,017.00 each |
|
| Akoya MOTiF Opal staining and scanning (IMC) |
FFPE slides stained with up to 6 antibodies using the Akoya MOTiF workflow using either Akoya panel or custom created antibody panel. Minimum of 10 slides required. |
Inquire | |
| Antibody titration for IHC/IF (IMC) |
Determine the optimal concentration and epitope retreival for whole slide imaging with a user-supplied antibody. A minimum of 6 conditions will be tested, cost is per round of testing. Typically, one round is sufficient to determine the optimal staining conditions. |
Inquire | |
| Automated slide staining (IMC) |
|
Internal
$20.75
each
External $33.00 each External Affiliate $27.00 each |
|
| Brightfield Scanning on Akoya PhenoImager HT (IMC) |
Core staff will scan your H&E or immunostained (DAB or similary monochromatic dye) slides. Digital qptiff files can be read using Akoya's Phenochart program, Leica's Imagescope or QuPath. |
Internal
$24.75
each
External $39.75 each External Affiliate $32.25 each |
|
| EVOS microscope (IMC) |
Assisted use of EVOS M500 microscope. Fluorescent channels include DAPI, GFP and Texas Red. Price is per hour of use and scheduled by Core based on availability. |
Inquire | |
| Fluorescent Scanning on Akoya PhenoImager HT (IMC) |
Core staff will scan fluorescently stained slides on the Akoya PhenoImager HT whole slide scanner. Digital files are in a qptiff format that can be viewed in Akoya's free Phenochart program or QuPath. |
Internal
$37.25
each
External $59.50 each External Affiliate $48.25 each |
|
| Four-plex antibody staining (IMC) |
Slides stained with 4 antibodies on separate fluorescent channels and counterstained with a nuclear dye. User provides primary antibodies which should show unique staining patterns in the specimen. Not all antibody combinations will be suitable for this 4-plex staining service.
Service requires a minimum of 4 slides. The core can advise users on performing this staining manually in their own lab.
|
Internal
$123.75
each
External $198.00 each External Affiliate $160.75 each |
|
| Four-Plex, 5-Color panel development (IMC) |
Core staff will create an assay to stain FFPE slides with 4 antibodies on separate fluorescent channels. |
Internal
$494.50
each
External $791.25 each External Affiliate $642.75 each |
|
| Nanostring Prep and nCounter (IMC) |
Prepare and run 12 samples on the nanostring nCounter bulk gene analysis platform. User provides the reagents and consumeables from nanoString. |
Internal
$309.00
each
External $494.50 each External Affiliate $401.75 each |
|
| Purify RNA from FFPE samples (IMC) |
RNA extraction from 12 FFPE samples for analysis with the nanoString nCounter instrument. |
Internal
$257.50
each
|
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| ► Accessories (5) | ||||||||||||||||||||||||||||||
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| ► Analyzer (9) | ||||||||||||||||||||||||||||||
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| ► Imager (1) | ||||||||||||||||||||||||||||||
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| ► Sorters (4) | ||||||||||||||||||||||||||||||
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