Gene Targeting/Gene Modification/Transgenic Mouse

Overview of Services

Gene Modification
The Gene Modification Facility is offering services for gene modification in cell lines and in conjunction with the Gene Targeting and Transgenic Mouse Facilities for the complete generation of gene targeted mouse lines including the generation of conventional gene knockouts, conditional knockouts, knock-in mutations and the generation of tagged proteins by conventional homologous recombination based gene targeting strategies or CRISPR (clustered regularly interspaced short palindromic repeats)-mediated gene targeting strategies. CRISPR can shorten the time frame of gene targeted mouse production remarkably and the gene targeted mice can be generated as fast as 2-4 months. Services for each individual step involved in the generation of gene targeted mouse lines are also offered.

Gene Targeting
The Gene Targeting Facility is a centralized facility for the production of gene-targeted mice. The services provided by the facility allow researchers to modify the mouse genome through methods of gene targeting or BAC transgenesis in embryonic stem (ES) cells and to introduce these changes into the mouse genome by the generation of targeted ES cells and chimeric mice. The facility also works in conjunction with the Gene Modification Facility to generate knockout mice.

Transgenic Mouse
The Transgenic Mouse Facility trains and assists Einstein investigators in the production and characterization of transgenic mouse strains. The personnel have more than a decade of experience providing high-quality service with minimal waiting times. The facility offers a wide array of mouse embryo-based services for the college, including traditional pronuclear and lentiviral injections as well as consulting, construct design and screening strategies.

Leadership

Scientific Director

Winfried Edelmann, PhD

718.678.1086

winfried.edelmann@einsteinmed.edu

Location and hours of operation

Gene Modification

Gene Targeting

Transgenic Mouse

Hours: Monday - Friday 9:00 AM - 5:00 PM

Location: Price 269

Location: Price Center/Block Research

Pavillion 269

Location: Price Center/Block Research Pavillion B110

Links and Resources

Contacts

Name	Role	Phone	Email	Location
Yongwei Zhang, PhD	Gene Modification Operations Director	718.678.1087	yongwei.zhang@einsteinmed.edu
	Gene Targeting Operations Director	718.678.1221, 718.678.1087		Price Center, Room B110
Ken Chen, M.D.	Transgenic Mouse Operations Director	718.678.1106	ken.chen@einsteinmed.edu	Price Center, Room B110

Services (Price List)

Name	Description	Price
Blastocyst Flushing	Blastocysts are flushed from day 3.5 mice. Each unit is one day of flushing. If multiple days are requested, adjust the number of Unit Qty as needed. Please bring your own flushing media, a minimum of 2ml per mouse is required.	Internal $220.00 each External $220.00 each External Affiliate $220.00 each
Blastocyst Injection		Inquire
Cage Fee (In Core) - Avg # of Cages per Day x # of Days	Fee is per day/per cage.	Inquire
Complete Gene Targeting Service -Complex targeting vector		Inquire
Complete Gene Targeting Service- Conditional Knockout targeting vector		Inquire
Complete Gene Targeting Service- Conventional Knockout targeting vector		Inquire
Complete Gene Targeting Service- Knockin vector		Inquire
Complex genetically engineered mouse production by zygote injection-C57BL/6* - PHASE I (A)	Including: Guide RNA design and prep Cas9 mRNA prep HR donor DNA design and prep	Inquire
Complex genetically engineered mouse production by zygote injection-C57BL/6* - PHASE I (B)	Including: Guide RNA design and prep Cas9 mRNA prep HR donor DNA design and prep	Inquire
Complex genetically engineered mouse production by zygote injection-C57BL/6* - PHASE I (C)	Including: Guide RNA design and prep Cas9 mRNA prep HR donor DNA design and prep	Inquire
Complex genetically engineered mouse production by zygote injection-C57BL/6* - PHASE II (A)	Including: Zygote injection Transgenic mouse production and genotyping	Inquire
Complex genetically engineered mouse production by zygote injection-C57BL/6* - PHASE II (B)	Including: Zygote injection Transgenic mouse production and genotyping	Inquire
Complex genetically engineered mouse production by zygote injection-C57BL/6* - PHASE II (C)	Including: Zygote injection Transgenic mouse production and genotyping	Inquire
Conditional(flox) allele generation by zygote injection-C57BL/6* - PHASE I	Including: Guide RNA design and prep Cas9 mRNA prep HR donor DNA design and prep	Inquire
Conditional(flox) allele generation by zygote injection-C57BL/6* - PHASE II	Including: Zygote injection Transgenic mouse production and genotyping	Inquire
Conventional KO mouse production by zygote injection-C57BL/6* - PHASE I	Phase I includes: Guide RNA design and prep Cas9 mRNA prep	Inquire
Conventional KO mouse production by zygote injection-C57BL/6* - PHASE II	Phase II includes: Zygote injection Transgenic mouse production and genotyping	Inquire
CRISPR DNA cleaning		Inquire
CRISPR injection		Inquire
CRISPR/Cas founder breeding for germline transmission		Inquire
CRISPR/Cas founder off- target analysis**, up to 3 founders, 10 off-target sites		Inquire
CRISPR/Cas mediated KO mammalian cell lines generation		Inquire
Design and cloning of HR Donor constructs for CRISPR/cas mediated genome engineering	Inquire about pricing.	Inquire
DNA cleaning for pronuclear injection		Inquire
Embryo cleaning	Per mouse. Note: Investigators must bring dissected oviducts.	Inquire
Embryo Cryopreservation		Inquire
Embryo Harvesting (per mouse)		Inquire
ES Cells Expansion		Inquire
Gene knockin	Inducible Cas9 + sgRNA; customer must provide homology template containing sequence to be knocked in and appropriate homology arms Cas9 expressed from pCW-Cas9 (Tet-ON, puromycin resistance) sgRNA expressed from pLX-sgRNA (U6 promoter, blasticidin resistance) An additional optimization charge of $150 per parental cell line may apply	Inquire
Gene Targeting Breeding		Internal $1,260.00 each External $1,890.00 each External Affiliate $1,575.00 each
Generation of inducible polyclonal Cas9/sgRNA cell line	sgRNA design and cloning Preparation of Cas9 and sgRNA lentivirus Transduction with Cas9 lentivirus and selection on antibiotic Wild-type Cas9 (for knockouts, deletions, mutations, and knockins) or dCas9-VP64 (for activation) Transduction with sgRNA lentivirus and selection on blasticidin Engineered cells delivered as frozen stocks Customer must induce Cas9 expression with doxycycline to effect gene editing or activation	Inquire
Generation of monoclonal KO cell line - sgRNA/Cas9 all-in-one plasmid based CRISPR Engineering	sgRNA design and cloning Transfection and short term selection on puromycin or cell sorting on mcherry Measure gene engineering efficiency with T7E1 assay or DNA sequencing Generation of monoclonal cell lines Identification of desired KO monoclonal cell lines by DNA sequencing Engineered cells delivered as frozen stocks	Inquire
Generation of monoclonal KO cell line - sgRNA/Cas9 all-in-one plasmid based CRISPR Engineering		Inquire
Generation of monoclonal KO cell line – sgRNA /inducible Cas9 lentivirus based CRISPR Engineering	sgRNA design and cloning Preparation of Cas9 and sgRNA lentivirus Transduction with Cas9 lentivirus and selection on antibiotic Transduction with sgRNA lentivirus and selection on blasticidin Induce Cas9 expression with doxycycline to effect gene editing Measure gene engineering efficiency with T7E1 assay or DNA sequencing Generation of monoclonal cell lines Identification of desired KO monoclonal cell lines by DNA sequencing	Inquire
Generation of polyclonal KO cell line – sgRNA /inducible Cas9 lentivirus based CRISPR Engineering	sgRNA design and cloning Preparation of Cas9 and sgRNA lentivirus Transduction with Cas9 lentivirus and selection on antibiotic Transduction with sgRNA lentivirus and selection on blasticidin Induce Cas9 expression with doxycycline to effect gene editing Measure gene engineering efficiency with T7E1 assay or DNA sequencing Engineered cells delivered as frozen stocks	Inquire
Genotyping	This service is per sample.	Inquire
gRNA-Cas9 constructs design and cloning for genome engineering of mammalian cell lines	Within 2 weeks	Inquire
gRNA-Cas9 constructs design and cloning for genome engineering of mammalian cell lines	Within 4 weeks.	Inquire
gRNA-Cas9 constructs design, cloning and validation for genome engineering of mammalian cell lines		Inquire
HyperOva Media	HyperOva is an enhanced superovulation reagent developed with the aim of inducing ovulation at a higher rate than possible following the conventional superovulation method (PMSG-hCG). Each unit charged is 1 mouse. Adjust Unit Qty based on the number of mice you need superovulated.	Internal $20.00 each External $20.00 each External Affiliate $20.00 each
In-vitro Fertilization		Inquire
KI (small modifications, <50bp) mouse production by zygote injection-C57BL/6* - PHASE I	Phase I includes: Guide RNA design and prep Cas9 mRNA prep HR donor DNA design and prep	Inquire
KI (small modifications, <50bp) mouse production by zygote injection-C57BL/6* - PHASE II	Phase II includes: Zygote injection Transgenic mouse production and genotyping	Inquire
Lentiviral Transgenesis using: BALB/c, C3h/HeJ, NOD/Lt		Inquire
Lentiviral Transgenesis using: FVB, C57/B6, or hybrid strain		Inquire
Mouse identification	per mouse - toe clipping - ear notch - cut tails	Inquire
Ovary Transplant		Inquire
Preparation of sgRNA/Cas9 all-in-one plasmid or sgRNA lentiviral construct (within 2 weeks)	sgRNA design and cloning	Inquire
Preparation of sgRNA/Cas9 all-in-one plasmid or sgRNA lentiviral construct (within 4 weeks)	sgRNA design and cloning	Inquire
Pronuclear injection using C57/B6 embryos		Inquire
Pronuclear injection using FVB embryos		Inquire
Rederivation		Inquire
Sperm Cryopreservation		Inquire
Superovulation Hormones (1 set PMS/HCG for 10 mice)		Inquire
Surcharge for using C57B6 background instead of 129 standard background		Inquire
Tail/Toe Clipping with Label (per mouse)		Inquire
Vasectomies (per mouse)		Inquire

Search available services: View: by category alphabetically

Name	Description	Price
▼ ► CRISPR/Cas Services (8)
Cage Fee (In Core) - Avg # of Cages per Day x # of Days	Fee is per day/per cage.	Inquire
CRISPR/Cas founder breeding for germline transmission		Inquire
CRISPR/Cas founder off- target analysis**, up to 3 founders, 10 off-target sites		Inquire
CRISPR/Cas mediated KO mammalian cell lines generation		Inquire
Design and cloning of HR Donor constructs for CRISPR/cas mediated genome engineering	Inquire about pricing.	Inquire
Genotyping	This service is per sample.	Inquire
gRNA-Cas9 constructs design and cloning for genome engineering of mammalian cell lines	Within 4 weeks.	Inquire
gRNA-Cas9 constructs design, cloning and validation for genome engineering of mammalian cell lines		Inquire
▼ ► CRISPR Cell Line Services (7)
Name	Description	Price
Gene knockin	Inducible Cas9 + sgRNA; customer must provide homology template containing sequence to be knocked in and appropriate homology arms Cas9 expressed from pCW-Cas9 (Tet-ON, puromycin resistance) sgRNA expressed from pLX-sgRNA (U6 promoter, blasticidin resistance) An additional optimization charge of $150 per parental cell line may apply	Inquire
Generation of inducible polyclonal Cas9/sgRNA cell line	sgRNA design and cloning Preparation of Cas9 and sgRNA lentivirus Transduction with Cas9 lentivirus and selection on antibiotic Wild-type Cas9 (for knockouts, deletions, mutations, and knockins) or dCas9-VP64 (for activation) Transduction with sgRNA lentivirus and selection on blasticidin Engineered cells delivered as frozen stocks Customer must induce Cas9 expression with doxycycline to effect gene editing or activation	Inquire
Generation of monoclonal KO cell line - sgRNA/Cas9 all-in-one plasmid based CRISPR Engineering	sgRNA design and cloning Transfection and short term selection on puromycin or cell sorting on mcherry Measure gene engineering efficiency with T7E1 assay or DNA sequencing Generation of monoclonal cell lines Identification of desired KO monoclonal cell lines by DNA sequencing Engineered cells delivered as frozen stocks	Inquire
Generation of monoclonal KO cell line – sgRNA /inducible Cas9 lentivirus based CRISPR Engineering	sgRNA design and cloning Preparation of Cas9 and sgRNA lentivirus Transduction with Cas9 lentivirus and selection on antibiotic Transduction with sgRNA lentivirus and selection on blasticidin Induce Cas9 expression with doxycycline to effect gene editing Measure gene engineering efficiency with T7E1 assay or DNA sequencing Generation of monoclonal cell lines Identification of desired KO monoclonal cell lines by DNA sequencing	Inquire
Generation of polyclonal KO cell line – sgRNA /inducible Cas9 lentivirus based CRISPR Engineering	sgRNA design and cloning Preparation of Cas9 and sgRNA lentivirus Transduction with Cas9 lentivirus and selection on antibiotic Transduction with sgRNA lentivirus and selection on blasticidin Induce Cas9 expression with doxycycline to effect gene editing Measure gene engineering efficiency with T7E1 assay or DNA sequencing Engineered cells delivered as frozen stocks	Inquire
Preparation of sgRNA/Cas9 all-in-one plasmid or sgRNA lentiviral construct (within 2 weeks)	sgRNA design and cloning	Inquire
Preparation of sgRNA/Cas9 all-in-one plasmid or sgRNA lentiviral construct (within 4 weeks)	sgRNA design and cloning	Inquire
▼ ► Conventional Gene Targeting Services (Complete Services) (5)
Name	Description	Price
Complete Gene Targeting Service -Complex targeting vector		Inquire
Complete Gene Targeting Service- Conditional Knockout targeting vector		Inquire
Complete Gene Targeting Service- Conventional Knockout targeting vector		Inquire
Complete Gene Targeting Service- Knockin vector		Inquire
Surcharge for using C57B6 background instead of 129 standard background		Inquire
▼ ► Gene Targeting (5)
Name	Description	Price
Blastocyst Injection		Inquire
Embryo cleaning	Per mouse. Note: Investigators must bring dissected oviducts.	Inquire
ES Cells Expansion		Inquire
Gene Targeting Breeding		Internal $1,260.00 each External $1,890.00 each External Affiliate $1,575.00 each
Mouse identification	per mouse - toe clipping - ear notch - cut tails	Inquire
▼ ► Transgenic Mouse Services (18)
Name	Description	Price
Blastocyst Flushing	Blastocysts are flushed from day 3.5 mice. Each unit is one day of flushing. If multiple days are requested, adjust the number of Unit Qty as needed. Please bring your own flushing media, a minimum of 2ml per mouse is required.	Internal $220.00 each External $220.00 each External Affiliate $220.00 each
CRISPR DNA cleaning		Inquire
CRISPR injection		Inquire
DNA cleaning for pronuclear injection		Inquire
Embryo Cryopreservation		Inquire
Embryo Harvesting (per mouse)		Inquire
HyperOva Media	HyperOva is an enhanced superovulation reagent developed with the aim of inducing ovulation at a higher rate than possible following the conventional superovulation method (PMSG-hCG). Each unit charged is 1 mouse. Adjust Unit Qty based on the number of mice you need superovulated.	Internal $20.00 each External $20.00 each External Affiliate $20.00 each
In-vitro Fertilization		Inquire
Lentiviral Transgenesis using: BALB/c, C3h/HeJ, NOD/Lt		Inquire
Lentiviral Transgenesis using: FVB, C57/B6, or hybrid strain		Inquire
Ovary Transplant		Inquire
Pronuclear injection using C57/B6 embryos		Inquire
Pronuclear injection using FVB embryos		Inquire
Rederivation		Inquire
Sperm Cryopreservation		Inquire
Superovulation Hormones (1 set PMS/HCG for 10 mice)		Inquire
Tail/Toe Clipping with Label (per mouse)		Inquire
Vasectomies (per mouse)		Inquire
▼ ► Archived Services (14)
Name	Description	Price
Generation of monoclonal KO cell line - sgRNA/Cas9 all-in-one plasmid based CRISPR Engineering		Inquire
gRNA-Cas9 constructs design and cloning for genome engineering of mammalian cell lines	Within 2 weeks	Inquire
Complex genetically engineered mouse production by zygote injection-C57BL/6* - PHASE I (A)	Including: Guide RNA design and prep Cas9 mRNA prep HR donor DNA design and prep	Inquire
Complex genetically engineered mouse production by zygote injection-C57BL/6* - PHASE II (A)	Including: Zygote injection Transgenic mouse production and genotyping	Inquire
Complex genetically engineered mouse production by zygote injection-C57BL/6* - PHASE I (B)	Including: Guide RNA design and prep Cas9 mRNA prep HR donor DNA design and prep	Inquire
Complex genetically engineered mouse production by zygote injection-C57BL/6* - PHASE II (B)	Including: Zygote injection Transgenic mouse production and genotyping	Inquire
Complex genetically engineered mouse production by zygote injection-C57BL/6* - PHASE I (C)	Including: Guide RNA design and prep Cas9 mRNA prep HR donor DNA design and prep	Inquire
Complex genetically engineered mouse production by zygote injection-C57BL/6* - PHASE II (C)	Including: Zygote injection Transgenic mouse production and genotyping	Inquire
Conditional(flox) allele generation by zygote injection-C57BL/6* - PHASE I	Including: Guide RNA design and prep Cas9 mRNA prep HR donor DNA design and prep	Inquire
Conditional(flox) allele generation by zygote injection-C57BL/6* - PHASE II	Including: Zygote injection Transgenic mouse production and genotyping	Inquire
Conventional KO mouse production by zygote injection-C57BL/6* - PHASE I	Phase I includes: Guide RNA design and prep Cas9 mRNA prep	Inquire
Conventional KO mouse production by zygote injection-C57BL/6* - PHASE II	Phase II includes: Zygote injection Transgenic mouse production and genotyping	Inquire
KI (small modifications, <50bp) mouse production by zygote injection-C57BL/6* - PHASE I	Phase I includes: Guide RNA design and prep Cas9 mRNA prep HR donor DNA design and prep	Inquire
KI (small modifications, <50bp) mouse production by zygote injection-C57BL/6* - PHASE II	Phase II includes: Zygote injection Transgenic mouse production and genotyping	Inquire