Macromolecular Therapeutics Development Facility (MTDF)

Generation of bacmids using Fast-bac or similar system

Small scale purification and qualitative expression analysis

Viral amplification through bacmid transfection or viral infection of Sf9 cells.

No cost to Einstein/Montefiore investigators for consultation on immunization, fusion & cloning, general cell culture or elisa & immunoassays.

charge per hour

(3 media, 3 strains)

*pricing is per target.

Immunization and titer of mice, fusion of one mouse, elisa screening, cloning, and freezing of clones.

Investigator must provide the antigen.

Thaw cell line, refreeze & adapt to serum-free media if needed prior to loading flask. Investigator must provide cells.

Investigator must provide the antigen.

Library screening of ligand binding to transiently transfected targets. 

Cost per 96 well plate

Price is per hybridoma.

Culture volume at least 5L

Price /5L unit

(PSAM52. LB, 2xYT) 

Quote is per liter culture volume.

Price/L

(PSAM52. LB, 2xYT) 

(15N labeling)

(13C,15N labeling)

Generation of lentivirus by transfection of 293T cells in 10cm plate

Small scale purification and qualitative expression analysis

Lentiviral production and titer by p24 ELISA 

Charge is for 48 DNA mini preps

Average yield of harvest- 0.5-1.5mg/ml secreted antibody.

Average yield of harvest- 0.5-1.5mg/ml secreted antibody.

Average yield of harvest- 0.5-1.5mg/ml secreted antibody. 

Average yield of harvest- 0.5-1.5mg/ml secreted antibody.

Thaw cell line, refreeze, collect large volume of supernatant using regular media. Investigator must provide cells.

*pricing is per column.

*pricing is per column

*pricing is per column

SDS-PAGE, protein identity by mass spec, endotoxin test (if required)

(SDS Page and Mass spectroscopy)

Recovery and culture of investigator provided transformed mammalian cell-line, including mycoplasma assay. Additional charge will be required for complex or unusual growth requirements.

Selection of a stable cell line pool through continuous growth in media with antibiotic. Periodic testing of the cell line for protein expression through small scale protein purification or FACS analysis.

*pricing is per target.

Price per 48 mutations within a single vector.

Cost may be increased depending on the length of the sequence to be mutagenized- contact the core for details.

price per 96 well plate

*pricing is per target.

Small scale (30ml) transient transfection, analysis by SDS-PAGE and SEC-MALS.

Transient mammalian expression or baculoviral infection of insect cells.

Affinity purification, Coomassie stained PAGE

Small scale expression validation of target by western blot, using either antibody directed against affinity tag, or investigator provided antibody.

Small scale expression validation of target by western blot, using either antibody directed against affinity tag, or investigator provided antibody. The charge for a single sample includes set-up fee.

24 well transient transfection.

1ml/well transient

$100/ target + cost/unit volume (~$500/L)

7 vectors to choose from

*pricing is per target.

Charge is for 48 DNA mini preps

Price per 48 mutations within a single vector.

Cost may be increased depending on the length of the sequence to be mutagenized- contact the core for details.

7 vectors to choose from

*pricing is per target.

*pricing is per target.

Generation of bacmids using Fast-bac or similar system

Viral amplification through bacmid transfection or viral infection of Sf9 cells.

Generation of lentivirus by transfection of 293T cells in 10cm plate

Lentiviral production and titer by p24 ELISA 

SDS-PAGE, protein identity by mass spec, endotoxin test (if required)

Recovery and culture of investigator provided transformed mammalian cell-line, including mycoplasma assay. Additional charge will be required for complex or unusual growth requirements.

Selection of a stable cell line pool through continuous growth in media with antibiotic. Periodic testing of the cell line for protein expression through small scale protein purification or FACS analysis.

Small scale (30ml) transient transfection, analysis by SDS-PAGE and SEC-MALS.

Small scale expression validation of target by western blot, using either antibody directed against affinity tag, or investigator provided antibody.

Small scale expression validation of target by western blot, using either antibody directed against affinity tag, or investigator provided antibody. The charge for a single sample includes set-up fee.

24 well transient transfection.

1ml/well transient

$100/ target + cost/unit volume (~$500/L)

Small scale purification and qualitative expression analysis

Transient mammalian expression or baculoviral infection of insect cells.

Affinity purification, Coomassie stained PAGE

Quote is per liter culture volume.

Small scale purification and qualitative expression analysis

*pricing is per column

*pricing is per column

Culture volume at least 5L

Price /5L unit

(PSAM52. LB, 2xYT) 

*pricing is per column.

(SDS Page and Mass spectroscopy)

price per 96 well plate

Price/L

(PSAM52. LB, 2xYT) 

(15N labeling)

(13C,15N labeling)

*pricing is per target.

(3 media, 3 strains)

*pricing is per target.

charge per hour

Library screening of ligand binding to transiently transfected targets. 

Cost per 96 well plate

No cost to Einstein/Montefiore investigators for consultation on immunization, fusion & cloning, general cell culture or elisa & immunoassays.

Immunization and titer of mice, fusion of one mouse, elisa screening, cloning, and freezing of clones.

Investigator must provide the antigen.

Thaw cell line, refreeze & adapt to serum-free media if needed prior to loading flask. Investigator must provide cells.

Investigator must provide the antigen.

Price is per hybridoma.

Average yield of harvest- 0.5-1.5mg/ml secreted antibody.

Average yield of harvest- 0.5-1.5mg/ml secreted antibody.

Average yield of harvest- 0.5-1.5mg/ml secreted antibody. 

Average yield of harvest- 0.5-1.5mg/ml secreted antibody.

Thaw cell line, refreeze, collect large volume of supernatant using regular media. Investigator must provide cells.