Welcome!
The Macromolecular Therapeutics Development Facility (MTDF) provides expertise for directed development and optimization of protein-based therapeutics and supports high-throughput automated development, analysis and production of protein reagents using a Biomek FxP liquid handling workstation for HTP molecular biology and expression testing, a Perkin-Elmer Cell::Explorer robot for very efficient HTP tissue culture based application and a custom microarray printer. This automation is designed to provide Einstein researchers with the added leverage of robotics equipment and trained personnel to accelerate and scale up their projects, thereby obviating the need for individual investigators to set up and staff their own facilities for protein production.
The Hybridoma Facility produces mouse monoclonal antibodies to antigens provided by investigators. For most investigators, the staff of the facility carries out the entire process from mouse immunization through fusion for hybridoma generation, cloning, screening, production of monoclonal antibody (mAb), and freezing of cell lines for long-term storage. In general, facility staff meet with the Principal Investigator, or his or her representative, to discuss the scientific goals of the project and to make decisions regarding immunogen preparation, immunization strategy, screening for positive hybridomas once a fusion is completed, and the types of antibodies required. Investigators provide the antigen for immunization and screening, while the facility provides and maintains the mice and performs all steps from immunization through fusion, cloning, and primary screening.
Steven C. Almo, Ph.D.
Scientific Director
steve.almo@einsteinmed.edu
Co-Scientific Director (Monoclonal Antibodies)
Matthew D. Scharff, M.D.
matthew.scharff@einsteinmed.edu
Steven Porcelli, M.D.
Scientific Director, Hybridoma Facility
steven.porcelli@einsteinmed.edu
| Hours | Location |
|
9 am - 5 pm Monday - Friday |
1301 Morris Park Ave Price Center, Room 313 Bronx, NY 10461 |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Scott J. Garforth, Ph.D. |
Operations Director
|
718-678-1146
|
scott.garforth@einsteinmed.edu
|
Price Center, Room 274
|
| Susan Buhl |
Associate Director, Monoclonal Antibodies
|
718-430-2170
|
susan.buhl@einsteinmed.edu
|
Chanin, Room 404
|
| Services |
| ► Eukaryotic (27) | |||
| Name | Description | Price | |
|---|---|---|---|
| Bacmid generation |
Generation of bacmids using Fast-bac or similar system |
Internal
$200.00
each
|
|
| Baculovirus amplification |
Viral amplification through bacmid transfection or viral infection of Sf9 cells. |
Inquire | |
| Baculovirus titering | Inquire | ||
| Lentiviral generation |
Generation of lentivirus by transfection of 293T cells in 10cm plate |
Internal
$100.00
each
External $200.00 each External Affiliate $150.00 each |
|
| Lentiviral generation and titer |
Lentiviral production and titer by p24 ELISA |
Inquire | |
| QC |
SDS-PAGE, protein identity by mass spec, endotoxin test (if required) |
Inquire | |
| QC with endotoxin testing | Inquire | ||
| Recovery and culture of mammalian cell-line |
Recovery and culture of investigator provided transformed mammalian cell-line, including mycoplasma assay. Additional charge will be required for complex or unusual growth requirements. |
Inquire | |
| Selection of a stable cell line |
Selection of a stable cell line pool through continuous growth in media with antibiotic. Periodic testing of the cell line for protein expression through small scale protein purification or FACS analysis. |
Inquire | |
| Small scale expression testing with SEC MALS |
Small scale (30ml) transient transfection, analysis by SDS-PAGE and SEC-MALS. |
Inquire | |
| Small scale expression validation, western- 2-10 samples. |
Small scale expression validation of target by western blot, using either antibody directed against affinity tag, or investigator provided antibody. |
Inquire | |
| Small scale expression validation, western- first sample. |
Small scale expression validation of target by western blot, using either antibody directed against affinity tag, or investigator provided antibody. The charge for a single sample includes set-up fee. |
Inquire | |
| TEV cleavage- eukaryotic expression | Inquire | ||
| Transient transfection |
24 well transient transfection. 1ml/well transient |
Inquire | |
| Transient transfection and growth ( |
$100/ target + cost/unit volume (~$500/L) |
Inquire | |
| Baculoviral generation and small scale expression validation of target |
Small scale purification and qualitative expression analysis |
Inquire | |
| Small scale expression validation |
Transient mammalian expression or baculoviral infection of insect cells. Affinity purification, Coomassie stained PAGE |
Inquire | |
| Large scale (P3) amplification of virus | Inquire | ||
| Large scale growth |
Quote is per liter culture volume. |
Inquire | |
| Large scale growth - Endotoxin-free protein production | Inquire | ||
| Large scale lentivirus expression: | Inquire | ||
| Large scale transient expression: | Inquire | ||
| Lentiviral generation and small scale expression validation of target |
Small scale purification and qualitative expression analysis |
Inquire | |
| Protein purification |
*pricing is per column |
Inquire | |
| Protein purification - Endotoxin-free protein production |
*pricing is per column |
Inquire | |
| Transfection Grade DNA preparation (Large scale): | Inquire | ||
| Transfection grade DNA preparation (small scale) | Inquire | ||
| ► Prokaryotic (12) | |||
| Name | Description | Price | |
| Deaggregation Assay | Inquire | ||
| Expression validation |
Soluble/insoluble expression testing on investigator-provided sample |
Inquire | |
| Large Scale Growth |
Culture volume at least 5L Price /5L unit (PSAM52. LB, 2xYT) |
Inquire | |
| Protein Purification |
*pricing is per column. |
Inquire | |
| Quality Control |
(SDS Page and Mass spectroscopy) |
Inquire | |
| Small Scale Expression and Validation |
price per 96 well plate |
Inquire | |
| Tag Removal | Inquire | ||
| Large Scale Growth - 1 |
Price/L (PSAM52. LB, 2xYT) |
Inquire | |
| Large Scale Growth - 2 |
(15N labeling) |
Inquire | |
| Large Scale Growth - 3 |
(13C,15N labeling) |
Inquire | |
| Small Scale Expression and Validation |
*pricing is per target. |
Inquire | |
| Expression Validation |
(3 media, 3 strains)
*pricing is per target. |
Inquire | |
| ► Hybridoma Facility (21) | |||
| Name | Description | Price | |
| Consultation for Hybridoma Projects |
No cost to Einstein/Montefiore investigators for consultation on immunization, fusion & cloning, general cell culture or elisa & immunoassays. Note that these fees will be waived if the client decides to proceed with mAb generation. For external projects, typically 1-2 hours of consultation required. |
Inquire | |
| Expansion and cryopreservation of hybridoma cell line | Inquire | ||
| Generation of Monoclonal Antibodies |
Immunization and titer of mice, fusion of one mouse, elisa screening, cloning, and freezing of clones. |
Inquire | |
| Generation of Monoclonal Antibodies with Investigator Assistance |
Investigator must provide the antigen. In this case, facility personnel perform immunization and fusion, but investigator takes over the major share of subsequent colony expansion and cloning using the prescreened facility reagents including FCS, media, ELISA reagents, agar for cloning etc. Continuing advice is provided at each step. |
Inquire | |
| High Antibody Concentration Supernatant |
Thaw cell line, refreeze & adapt to serum-free media if needed prior to loading flask. Investigator must provide cells. |
Inquire | |
| High Antibody Concentration Supernatant Quantitation by Elisa | Inquire | ||
| Immunization and titer of 5 mice |
Investigator must provide the antigen. |
Inquire | |
| Isotype Switching |
Price is per hybridoma. |
Inquire | |
| mAb Isotype determination | Inquire | ||
| mAb production in 1000 ml Bioreactor flask | Inquire | ||
| mAb production in 350 ml Bioreactor flask | Inquire | ||
| Measurement of mAb concentration by ELISA | Inquire | ||
| Production of High Antibody Concentration Supernatant in Integra Flask Using DME/FCS (45-50ml) |
Average yield of harvest- 0.5-1.5mg/ml secreted antibody. |
Inquire | |
| Production of High Antibody Concentration Supernatant in Integra Flask Using DME/FCS (80-90ml) |
Average yield of harvest- 0.5-1.5mg/ml secreted antibody. |
Inquire | |
| Production of High Antibody Concentration Supernatant in Integra Flask Using Serum-Free Media (45-50ml) |
Average yield of harvest- 0.5-1.5mg/ml secreted antibody. |
Inquire | |
| Production of High Antibody Concentration Supernatant in Integra Flask Using Serum-Free Media (80-90ml) |
Average yield of harvest- 0.5-1.5mg/ml secreted antibody. |
Inquire | |
| Production of Normal Antibody Concentration Supernatant |
Thaw cell line, refreeze, collect large volume of supernatant using regular media. Investigator must provide cells. |
Inquire | |
| Purification of IgG mAbs | Inquire | ||
| Recovery of cryopreserved hybridoma | Inquire | ||
| Standard hybridoma culture supernatant, large volume (~500 ml) | Inquire | ||
| Standard hybridoma culture supernatant, medium volume (~50 ml) | Inquire | ||
| ► Molecular Biology (6) | |||
| Name | Description | Price | |
| Miniprep, 96 well format |
Charge is for up to 48 DNA mini preps |
Inquire | |
| Site Directed Mutagenesis, 96-well plate format |
Price per 48 mutations within a single vector. Cost may be increased depending on the length of the sequence to be mutagenized- contact the core for details. |
Inquire | |
| Cloning (single target, standard LIC vector) | Inquire | ||
| Cloning (restriction cloning) | Inquire | ||
| Vector screening suite |
7 vectors to choose from
*pricing is per target. |
Inquire | |
| Site Directed Mutagenesis |
*pricing is per target. |
Inquire | |
| ► Other Services (7) | |||
| Name | Description | Price | |
| Analytical IEX | Inquire | ||
| Analytical IP and SDS-PAGE | Inquire | ||
| Consultation/development |
charge per hour |
Inquire | |
| Envision Plate Reader | Inquire | ||
| Intellicyt screening |
Library screening of ligand binding to transiently transfected targets. Cost per 96 well plate |
Inquire | |
| SEC MALS Analysis (Samples 1 to 5) | Inquire | ||
| SEC MALS Analysis (Samples 6 and Up) | Inquire | ||
